Isolation and Identification of Soil-borne Fungi in Some Agricultural Soils in Misurata City-Libya.

Abstract

This study was carried out in the Microbiology laboratory at faculty of science, Misurata University, to isolate and identify the soil-borne fungi in the agricultural soils of three areas in Misurata city these including: Alsaket, Aldafnaya, Tomina. The obtained results depending on the morphological characteristics of the fungal cultures and using the compound light microscope with assist of the modern scientific references showed that the soils contained many fungal genera, these are: Fusarium,  Alternaria, Aspergillus, Rhizopus and  Penicillium using two different fungal culture media: Potatoes Dextrose Agar (PDA) and Czapek Dox Agar (CDA)  and the serial dilution method at the concentrations of  〖10〗^(-2) and  〖10〗^(-3) . the isolated fungi were observed with various percentages which reached as high as 68.08% such as in genus of Penicillium sp. in Alsaket area on PDA at 〖10〗^(-3), whereas the Alternaria alternata was the lowest observed genus with percentage of 5%  in the same area  (Alsaket) and culture media (PDA), but at the concentration of  〖10〗^(-2). instead of 〖10〗^(-3) . Some fungal genera, to some extent were highly observed in some agricultural areas, while they were never observed in the same areas. The culture media PDA and CDA also had a key role to isolate the soil-borne fungi giving different percentages of the fungal isolates at the two tested dilutions 〖10〗^(-2) and 〖10〗^(-3) in the three studied areas Alsaket, Aldafnaya  and Tomina.