Influence of Gibberellic Acid (GA3) and kinetin (Kin) on in vitro regeneration of Salvia fruiticosa Mill.

Abstract

An in vitro cultivation protocol was developed for Salvia fruiticosa Mill, a species threatened by over collection due to its importance as a medicinal plant in Al-Jabal Al-Akhdar area, Libya.  The aim of this work is to establish an effective protocol for producing S, fruiticosa in vitro, which would contribute to resolving the state of secondary dormancy and conservation this species. Effect of auxin and Cytokinins   on seed germination percentage, morphological plant characters (plant length and seed vigor index) and photosynthetic pigments (Chlorophyll a and b) of seeds cultured on half-salt strength MS medium supplemented with 0.05 mg L-1 Gibberellic acid (GA3) and 0.05 mg L-1 kinetin (Kin). GA3 showed significant increase of germination percentages (55%) compared to Kin (20%). Highest seed vigor index was achieved with kin (53.93) compared to (12.55) with GA3.  There were no statistically significant differences differences in the plant length between different treatments. Chlorophyll (a) and (b) in leaves were high significantly increased with Kin compared to GA3         .